AcubeSAT is a student-led space biology mission developed by SpaceDot, a multidisciplinary team formed mostly by students of the Aristotle University of Thessaloniki, Greece. As part of ESA’s Fly You Satellite! programme, the team conducted a radiation test campaign at ESA ESTEC to help prepare their CubeSat for the harsh space environment. This milestone marks an important step forward in AcubeSAT’s development.